- GLSEN Tennessee works to create safe schools and provides leadership opportunities for youth.
- Launch Pad is a volunteer-based initiative striving to provide street-free sleep to youth between the ages of 18-24 with a focus on being affirming and welcoming to LGBTQ youth.
- The Music City Sisters raise money for HIV/AIDS organizations, fight for queer rights and visibility, and do safer-sex outreach.
- Nashville CARES provides support services to individuals and families affected by HIV and AIDS.
- Nashville LGBT Chamber of Commerce advances common business interests, economic growth, and equality in the workplace and society for its members.
- Nashville Pride is the nonprofit host of the annual Nashville Pride Festival, taking place annually in June.
- Oasis Center Just Us Program offers a variety of programs to help young LGBTQ people achieve their full potential.
- PFLAG Nashville provides free support for parents, family, and friends of lesbian, gay, bisexual, and transgender people.
- Tennessee Equality Project advocates for the equal rights of LGBTQ people in Tennessee.
- Trans Lifeline is a national trans-led organization dedicated to improving the quality of trans lives by responding to the critical needs of our community with direct service, material support, advocacy, and education.
- The Trevor Project is the leading national organization providing crisis intervention and suicide prevention services to lesbian, gay, bisexual, transgender, queer & questioning (LGBTQ) young people under 25.
- TVALS is Middle Tennessee’s transgender support group.
- Vanderbilt University Office of LGBTQI Life is a cultural center and a place of affirmation for individuals of the Vanderbilt community, and a resource for information and support about gender and sexuality.